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KMID : 0381020100430040367
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Korean Journal of Nutrition
2010 Volume.43 No. 4 p.367 ~ p.373
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Anti-Oxidative and Anti-Inflammatory Effect of Water Extract from Perillae semen in RBL-2H3 Cells
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Kim Jeong-Mi
Kim Dae-Jung
Kim Tae-Hyuk
Kim Hyun-Sook
Choe Myeon
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Abstract
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The present study was to investigate anti-oxidative and anti-inflammatory activity of Perillae semen in RBL-2H3 basophilic leukemia cells. Inhibitory effect of Perillae semen onto free radical generation was determined by measuring DPPH and hydroxyl radical scavenging activities in vitro. Anti-inflammatory actions of Perillae semen extracts (100, 250, 500 μg/mL) were assessed by testing their effects on the degranulation of mast cells. For this, β-hexosaminidase released from RBL-2H3 cells was used and proinflammatory cytokines were measured by an ELISA kit. Our results indicated that Perillae semen water extracts effectively inhibited free radical generation. At the concentration of 500 μg/mL of water extract, the degranulation of RBL-2H3 cells were inhibited by 42.1%. The IgE-antigen complex increased the accumulation of IL-4 and TNF-α secretion in RBL-2H3 cells and treatments with 250 and 500 μg/mL of Perillae semen extracts suppressed the IgE induced secretion of IL-4 and TNF-α protein by 20.5, 26.9% and 14.5, 16.5% respectively. We observed that Perillae semen water extract reduced β-hexosaminidase, IL-4, and TNF-α secretion in RBL-2H3 cells. These results provide that Perillae semen may be beneficial in the treatment of allergic inflammatory disease.
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KEYWORD
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perillae semen, RBL-2H3, anti-allergic, β-hexosaminidase, IL-4, TNF-α
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